Electronic Resource
New York, NY [u.a.]
:
Wiley-Blackwell
American Journal of Anatomy
118 (1966), S. 135-161
ISSN:
0002-9106
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
Fat absorption and transport in rat intestinal epithelial cells were followed by electron microscopy and radioautography. Physiological fatty-chyme, prepared from fat-fed donor rats, was injected into ligated segments of the upper jejunum of fasting recipient animals. Samples of tissue were removed in a time sequence from 1 to 30 minutes. Oleic acid-H3 was added to the chyme for light and electron microscopic radioautography. The results indicate that fat is absorbed and transported through the epithelium very rapidly. Within 1 minute, fat droplets can be seen in the endoplasmic reticulum and the Golgi apparatus. Fat is shown to be absorbed during, not prior to, experimentation by the radioautographic identification of labeling in the vicinity of fat droplets throughout the cell. Evidence of accumulation of fat within the Golgi apparatus appears between 1 and 2 minutes. Such accumulation may be indicative of a rate-limiting step in absorption. It is suggested that phenomena occurring within the Golgi apparatus are in part responsible for the appearance of cellular saturation with fat, which is evident in 8 to 10 minutes. The sequential pattern can be followed in cells along the entire length of the villus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/aja.1001180108
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