ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
Adult virgin females were made pseudopregnant by cervical stimulation and four days after stimulation the uteri were traumatized with a thread. The animals were autopsied 24, 48, 72, 96, and 120 hours after trauma. Frozen sections of the deciduomata were sectioned on the cryostat. For the identification of phosphorylase the sections were placed in the substrate containing 50 mg glucose-1-phosphate (G-1-P), 10 mg muscle adenylic acid, 15 ml distilled water, 10 ml 0.2 M acetate buffer (pH 5.8), 25 units of insulin and 5 mg of glycogen. To demonstrate uridinediphosphoglucose (UDPG)-glycogen transferase sections were placed in the substrate containing 50 mg UDPG, 10 mg glycogen, 20 mg Versene, 10 mg G-6-P, 14 ml distilled water, 10 ml 0.2 M tris buffer (pH 7.4) and 1 ml absolute alcohol. Following incubation for one hour at 37° the sections were treated with either Lugol's or Gram's solution until the color developed. A positive response for phosphorylase was a blue-black color while a red-purple color indicated UDPG-glycogen transferase. The periodic-leucofuchsin technique was used to identify glycogen and hemotoxylin and eosin sections were studied for general morphology.Glycogen and phosphorylase were observed in the decidual cells. However, the enzymatic activity was not as intense or as extensive as the polysaccharide. UDPG-glycogen transferase activity was observed in only two of the 50 deciduomata studied. On the basis of the present observations it may be suggested that the important pathway for glycogen synthesis in the decidual cells is from G-1-P by the action of phosphorylase and not from UDPG by the action of UDPG-glycogen transferase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091500208
