ISSN:
0003-276X
Schlagwort(e):
Life and Medical Sciences
;
Cell & Developmental Biology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Medizin
Notizen:
Light and electron microscopic analysis of adenovirus-infused ileal loops disclosed virus particles within the tubulo-vacuolar apparatus of absorptive cells. Tissue observed from five-minute loops contained virus particles largely within apical tubules and vesicular profiles. Similar particles were observed within cisternae and supranuclear vacuoles as well as within apical tubules in tissue samples from 10- and 15-minute loops. Loops maintained for 30 minutes demonstrated virions predominantly in the supranuclear vacuole of each absorptive cell. In no instance was a virion seen free within the cytoplasm or nucleus and none was apparent elsewhere in the mucosa. It appears that absorptive cells of the ileum are capable of sequestering exogenous virions and conveying them to established lysosomal bodies in the supranuclear cytoplasm. Here they may be either stored or degraded, thus impeding their progress to other intra- or extracellular locations. The possible significance of these findings is discussed in detail, and it is proposed that sequestration of ingested virions by the tubulo-vacuolar apparatus of the ileal absorptive cell may serve some protective function in the developing neonate. It is suggested that direct infusion of “purified” virus into intestinal loop preparations is an efficient means of observing virus-intestinal cell interactions in the living animal. This experimental model might prove effective in future studies of viral infection in the intestine, particularly if it is combined with both serological and morphological methods.
Zusätzliches Material:
4 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/ar.1091750105
