ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Cultures of BHK2l cells continuously release into serum-free medium, active materials which stimulate the incorporation of thymidine in non-confluent, but not in confluent cultures of homotypic cells. The activity is not removed by centrifugation at 30,000 g for four hours, but is non-dialysable and retained by membranes with a nominal limitation for MW 25,000. Activity is stable at 4° for several weeks, but destroyed in 30 minutes at 56°. BHK2l cells also release active material capable of enhancing the effect of added serum. This is also heat labile and its action is density-dependent.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1040780304
