ISSN:
0021-9541
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Purine nucleotides and nucleosides were found to enhance the DNA synthesis in quiescent 3T3 cells induced either by fresh medium containing calf serum or by the addition of the β-globulin serum fraction to depleted medium. A two to threefold enhancement has been obtained under these conditions. The adenine and hypoxanthine based compounds were more effective than the guanine ones in the concentration range tested (10-5--10-3 M), while the pyrimidine compounds were without effect. The degree of enhancement obtained with the purine compounds depended on the type and amount of serum used to stimulate DNA synthesis. A maximal degree of enhancement was obtained when the purine compounds were present for only the first three hours after the addition of serum and thus 18-20 hours before the actual onset of DNA synthesis.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1040810306
