ISSN:
0741-0581
Keywords:
Shadowing
;
Electron microscopy
;
Freeze fracture
;
Quick freezing
;
Mast cells
;
Neutrophils
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
Rotary shadowing when combined with such specimen preparation techniques as quick freezing and deep etching, critical point drying, and glycerol spraying is a highly versatile method of visualizing cell and macromolecular ultrastructure. This review outlines the procedures commonly used to prepare specimens for rotary shadowing and evaluates the relative merits of rotary shadowing when compared to unidirectional shadowing and negative staining.
Additional Material:
24 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060030304
