ISSN:
0741-0581
Keywords:
Small intestinal morphology
;
Immunohistochemistry
;
Electron microscopy
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
In the present study, we modified the technique described by Altman et al. (J. Histochem. Cytochem., 32:1217-1223, 1984) for rapid embedding of tissues in Lowicryl K4M. To attain good sections of the small intestine that contained villi, crypts, submucosa, and external muscle layers, we cut 100 μm slices of the full thickness of the wall with a vibratome before embedment and then deoxygenated the resin and tissue before polymerization. The sections we obtained compared favorably with the quality of sections from conventional resins.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060160106
