ISSN:
0095-9898
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
Dry weight, total protein, chlorophyll a, carotenoids, soluble protein, RNA, DNA, and total phosphorus were determined at intervals of two hours during synchronous growth of Euglena at a cell concentration of 5-10 × 104 cells/ml maintained by periodic dilution. Detailed analyses of the soluble proteins (DEAE-cellulose fractionation) and of the intracellular distribution of phosphorus were made also. In general, a linear doubling of each of these major components occurred during the light period; there was no net synthesis during the dark. Since cell number doubled during the dark period, a halving of the amounts of the parameters in each cell occurred. The most notable exception was the stepwise synthesis of DNA during the light period: DNA replicated during the last 6 hr only of the light period, commencing at 8 hr after the onset of light. Although DNA replication was a necessary condition for cell division to occur, it was not necessarily a sufficient one. It was found that different absolute rates of synthesis exist for the different compounds, with a doubling of a given parameter sometimes being completed before the end of the light period.
Additional Material:
15 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcp.1030660205