ISSN:
0192-253X
Schlagwort(e):
soybean
;
heat-shock gene
;
CaMV promoter
;
plant transformation
;
Life and Medical Sciences
;
Genetics
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
Notizen:
Transcription of heat-shock protein genes in soybean can be induced by high temperature stress leading to a transient expression of heat-shock proteins. We have tested whether the replacement of a native heat-shock promoter by a viral promoter results in constitutive transcript levels of the respective gene in transgenic plants. The 35S-transcript promoter of the cauliflower mosaic virus was linked to the protein-coding region of the genomic heat-shock gene hs6831, encoding a 17.6-kD heat-shock protein of soybean. After transformation of tobacco plants with this chimeric construction using a disarmed Agrobacterium binary vector, abundant mRNA levels were detected in transgenic plants. The steady-state level of this mRNA at 25°C was equal to that generated by the native heat-shock promoter at 40°C; however, it was markedly reduced by heat shock applied to the transgenic plants. These findings suggest a sufficiently high stability of heat-shock mRNA produced at the normal growth temperature to direct constitutive expression of heat-shock proteins. The application of constitutive gene expression for the investigation of thermotolerance is discussed.
Zusätzliches Material:
4 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/dvg.1020080507
