ISSN:
0192-253X
Keywords:
Homeobox
;
even-skipped
;
evel
;
no tail ntl
;
pattern formation
;
anterior-posterior axis
;
zebrafish embryo
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The zygotic expression of the evel gene is restricted to the ventral and laletul cells of the marginal zone. At later stages, the mRNAs are localized in the most posterior part of the extending tail tip. An evel clone (pcZf14), containing a poly-A tail, has been isolated. In order to address evel gene function, pcZf14 transcript injections into zebrafish embryos have been performed. The injection into uncleaved eggs of a synthetic evel mRNA (12 pg), which encodes a protein of 28 kd, produces embryos with anterior-posterior (A-P) axis defects and the formation of additional axial structures. The first category of 24 h phenotypes (87%) mainly displays a gradual decrease in anterior structures. This is comparable to previous phenotypes observed following Xhox3 messenger injection either in Xenopus or in zebrafish that have been classified according to the index of axis deficiency (zf-IAD). These phenotypes result in anomalies of the development of the neural keel, from microphthalmia to acephaly. The second category (13%) corresponds to the phenotypes described above together with truncal or caudal supernumerary structures. Additional truncal structures are the most prominent of these duplicated phenotypes, displaying a “zipper” shape of axial structures including neural keels and noto-chords. Caudal duplication presents no evident axis supernumerary structures. The observation of these phenotypes suggests an important role for the evel gene in mesodermal cell specification and in the development of the posterior region, and more particularly of the most posterior tail tip where endogenous eve1 messengers are found. © 1995 Wiley-Liss, Inc.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/dvg.1020170204
