ISSN:
0538-8066
Keywords:
Chemistry
;
Physical Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The Modified Simplex Method (MSM) coupled with contour diagrams is used to determine the rate constants of a kinetic scheme involving three sequential second order processes.Reaction between glutathione and the dithiocarbamate fungicide tetramethylthiuram disulfide produces the oxidized form of glutathione which is then reduced in the presence of NADPH. The concentration of the reducing agent is monitored as a function of time. In the absence of simplifying assumption a closed form solution of the rate equations does not exist. Computed curves of NADPH concentration versus time were generated using the software SIMULCIN.The rate constant values are optimized by the MSM procedure to obtain the best agreement between calculated and experimental data. The significance, validity limits of the estimated rate constants and the relevance of these in vitro data to previous in vivo studies are discussed.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/kin.550201102
