ISSN:
0018-019X
Keywords:
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Aldolase A (rabbit muscle) and aldolase B (human liver) have been resolved by isoelectric focusing into five distinct enzymatically active compounds. Each single component, if refocused under the same conditions, yields one single peak at the same isoelectric position as in the previous run. If component I or III are rehybridized (method of Penhoet et al.) component I yields one single peak, whereas component III yields the complete five membered set.Apparently the aldolases A and B are both tetrameres of two heterogeneous types of subunits α/α′ and β/β′ respectively. Analogous to the five membered set produced by hybridization of the aldolases A, B and C, the subunits α/α′ or β/β′, respectively, combine randomly to tetrameres forming five membered sets of the type α4, α3α′, α2α2′, α1α3′, α4′, etc.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/hlca.19700530326
