ISSN:
0018-019X
Keywords:
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The chemical synthesis of adenylyl-(2′-5′)-adenylyl-(2′-5′)-8-azidoadenosine (15) was performed by the phosphotriester approach. Enzymatic phosphorylation of 15 by [γ-32P]ATP led to the corresponding labelled 5′-monophosphate 16. Photoinsertion of 16 took place on UV irradiation by covalent cross linking to a protein of Mr 80 K known to be RNase L. Radiobinding and core-cellulose assays as well as photoaffinity labelling experiments with 16 are described.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/hlca.19890720624