ISSN:
0025-116X
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
The development of molar mass distribution of poly(D-(-)-3-hydroxybutyrate) (PHB) was studied during the intracellular synthesis in Alcaligenes eutrophus H16 bacteria with fructose as substrate and ammonium sulfate as nitrogen source. The flask culture fermentation experiments were carried out with a high cell density preculture under polymer synthesis conditions at different temperatures, pH values and substrate concentrations. These parameters were kept constant throughout the fermentation process. Polymer samples were isolated from cells by extraction with chloroform. The molar mass distribution of PHB was determined by gel-permeation chromatography in chloroform. A simple kinetic model based on Michaelis-Menten kinetics is postulated for synthesis of PHB. With this model the molar mass distributions of PHB were calculated and fitted to the experiment by variation of the kinetic constants.
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/macp.1993.021940712
