ISSN:
1420-908X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Enzymatically dispersed rat gastric cells were subdivided (Percoll®) in fractions (F1, F2, F3) with different number of parietal cells (PC) and the intracellular histamine content was estimated (ng/106 cells): F1 (9% PC): 17±4 (SEM), F2 (26% PC): 80±9 and F3 (77% PC): 134±15. Histidinedecarboxylase showed the same pattern of distribution, F1 low, F2 medium, F3 high activity. Incubated F3 cells constantly released histamine (19±3 ng/106 cells/h), a process which could be stimulated by carbachol, forskolin and hexoprenaline. The data suggest: (1) rat gastric histaminocytes copurify with PC, (2) background stimulation by histamine should be considered in isolated cell systems, (3) vagal stimulation may release histamine within the gastric mucosa.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01983139
