ISSN:
1420-9071
Keywords:
Aflatoxin
;
Aspergillus parasiticus
;
sclerotia
;
mycelial maceration
;
strain degeneration
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Serial transfer of mycelial macerates of a wild type, haploid, aflatoxigenic strain ofAspergillus parasiticus in a defined liquid medium resulted in the production of three new morphological classes: a sclerotial form with high aflatoxin production, and two variant forms (fan andfluff) with lowered sporulation, no sclerotia, and attenuated levels of aflatoxin. A genetically marked diploid containing mutant markers for aflatoxin pathway intermediates yielded the same three morphological classes upon serial transfer of macerated mycelia. When these diploid variants were treated with a haploidization agent, and the phenotypes of the resultant segregants scored, a low frequency of colonies producing aflatoxin pathway intermediates was recovered. These genetic data indicate that the structural genes for the aflatoxin pathway are present but somehow attenuated in thefan andfluff strains.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01941550
