ISSN:
1420-9071
Keywords:
Electronmicroscopy
;
laser
;
embryogenesis
;
mitosis
;
germline
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary In order to study development of embryos ofCaenorhabditis elegans at an ultrastructural level, a new method of fixation has been developed. With a laser microbeam coupled to a microscope the impermeable eggshell is punctured to allow penetration of the fixative. At specific stages of embryogenesis further development can be arrested at will under visual control. As fixation occurs instantaneously, transient events (e.g. different phases of mitosis and cytokinesis) can be visualized.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01940725