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Digitale Medien

Investigations of receptor-mediated phagocytosis by hormone-induced (imprinted)Tetrahymena pyriformis (1996)

Kovacs, P. ; Sundermann, C. A. ; Csaba, G.

Springer

Cellular and molecular life sciences 52 (1996), S. 769-773

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Details

ISSN: 1420-9071

Schlagwort(e): Insulin ; imprinting ; Tetrahymena ; phagocytosis ; insulin receptor

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Abstract Receptor-mediated endocytosis byTetrahvmena pyriformis was studied using tetramethylrhodamine isothiocyanate-labeled concanavalin A (TRITC-Con A) with fluorescence and confocal microscopy. In the presence of insulin, or 24 h after insulin pretreatment (hormonal imprinting), the binding and uptake of TRITC-Con A increased when compared to controls, owing to the binding of TRITC-Con A to sugar oligomers of insulin recptors. Mannose inhibited the binding of Con A, thus demonstrating the specificity of binding. Histamine, a phagocytosis-promoting factor in mammals andTetrahymena, and galactose, did not influence the uptake of TRITC-Con A.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01923987

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