ISSN:
1573-4986
Keywords:
gas chromatography-mass spectrometry
;
glycosphingolipids
;
liquid chromatography -NMR spectroscopy
;
pig
;
xenotransplantation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Glycosphingolipids were prepared from pig lung and pooled into two fractions with (i) ≥3 sugar residues, and (ii) ≤3 sugar residues. Oligosaccharides were prepared and used for gas chromatography, gas chromatography-mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry. The glycolipid fractions i and ii were further characterised and purified using a novel method based on high performance liquid chromatography “on-flow” proton nuclear magnetic resonance. The LC “on-flow” NMR technique showed good chromatographic separation and gave NMR spectral information which could be used as guidance for pooling of the separated mixture glycolipids. Conventional 1H NMR, thin layer immunostaining, gas chromatography, gas chromatography/mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry were used to characterise the glycolipids and to validate LC-NMR spectral data.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006901803636