ISSN:
1573-4838
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Technology
Notes:
Bioactive materials such as hydroxyapatite ceramics are known to show a stable interfacial bond with tissues. The objective of this study was to investigate the behaviour of human bone differentiated cells isolated from bone marrow on the surface of a hydroxyapatite ceramic obtained from bovine spongiosa. Scanning electron microscopy and DNA synthesis analysis assessed by [3H]-thymidine incorporation showed cell colonization of the whole material. Immunological studies using monoclonal antibodies to osteocalcin and osteonectin and cytochemical analysis of alkaline phosphatase activity indicated that these cells did not lose their osteoblastic phenotype after 28 days of culture. Furthermore, this study demonstrates the in vitro interface between the material and human cells, which is reporduced in vivo.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00120345