ISSN:
1572-9699
Keywords:
trehalases
;
vacuoles
;
glycoproteins
;
Candida utilis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The isolation of vacuoles by density gradient centrifugation of protoplast lysates fromCandida utilis cells showed a high specific activity for nonregulatory trehalase in vacuoles whereas the regulatory trehalase activatable by phosphorylation behaves as a cytoplasmic enzyme. The vacuolar trehalase is a glycoprotein that can be precipitated by Con A-Sepharose. Treatment of this enzyme with endo H reduced its reactivity with the lectin without loss of enzyme activity and decreased its apparent molecular weight by gel filtration.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00588391