ISSN:
1572-9699
Keywords:
Aspergillus
;
gene expression
;
heterologous protein
;
protein secretion
;
Trichoderma
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Despite the naturally high capacity for protein secretion by many species of filamentous fungi, secteted yields of many heterologous proteins have been comparatively low. The strategies for yield improvement have included the use of strong homologous promoters, increased gene copy number, gene fusions with a gene encoding a naturally well-secreted protein, protease-deficient host strains and screening for high yields following random mutagenesis. Such approaches have been effective with some target heterologous proteins but not others. Approaches used in heterologous protein production from filamentous fungi are discussed and a perspective on emerging strategies is presented.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00871952
