ISSN:
1573-5001
Keywords:
13C-labeled oligonucleotides
;
Triple helix
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary We present NMR studies of an intramolecular triple helix, the three strands of which have been linked by a hexaethylene glycol chain. To overcome the generally encountered difficulties of assignment in the homopyrimidine strands, the carbon C1′ of the pyrimidines were selectively 13C-enriched. Assignments of the aromatic and sugar protons were obtained from NOESY-HMQC and TOCSY-HMQC spectra. We show that the recognition of a DNA duplex by a third strand via triplex formation is easily carried out in solution by observing the changes of the 1H1′−13C1′ connectivities as a function of pH. Furthermore, the conformation of the sugars has been found to be C2′-endo, on the basis of the coupling constant values directly measured in an HSQC spectrum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00156621
