ISSN:
1573-8280
Keywords:
bone marrow
;
peripheral blood
;
cord blood
;
ex vivo expansion
;
haemopoietic stem cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Human pluripotential stem cells (PSC) are currently the target fortransplantation attempts and genetic manipu-lation. We have thereforeinvestigated the frequency and the expansion potential of PSC's in differenttypes of blood samples. CD 34+ cells were thus obtained from human bonemarrow (BM), as well as from peripheral blood (PB) and cord blood (CB)samples. After immuno-magnetic separation the highest yields of CD 34+cells were from BM (1.08-2.25%) and CB (0.42-1.32%) while PBsamples gave much lower values. Suspension cultures of PSC's from the threesources were then set up, in the presence of combinations of haemopoieticgrowth factors. A remarkable amplification of the nucleated cell pool wasobserved reaching a maximum between 10 and 15 days of culture; earliest andmaximum expansion (up to 220-fold) was achieved when Erythropoietin (Epo)was added to the culture medium, but this resulted in reduction ofcolony-forming cells and differentiation into erythroid progenitors.Clonogenic tests for BFU-E's derived colonies showed a peak value at 5 daysof liquid culture. Further studies are advisable to establish the bestcytokine combination for a valuable ex vivo expansion, coupled withpreservation of stem cell properties.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008081708054
