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Development of a shuttle vector for screening of strong promoter sequences (1999)

Tangney, Martin ; Mitchell, Wilfrid J. ; Dolberg-Rasmussen, Michael

Springer

Biotechnology techniques 13 (1999), S. 141-144

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ISSN: 1573-6784

Keywords: promoter probe vector ; gene expression ; transcriptional terminator

Source: Springer Online Journal Archives 1860-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: Abstract A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1023/A:1008983208118

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