ISSN:
1573-6784
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary A novel application of immobilized enzymes is presented. Cofactor dependent immobilized enzymes can be used as affinity chromatography supports to bind their substrates without alteration in absence of the cofactor. The procedure has been demonstrated using β-galactose dehydrogenase to retain D-galactose selectively in the absence of NAD. The uses of the technique are discussed from the down-stream processing and mechanistic points of view.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02447487