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A fast and efficient method for screening of nested deletions using PCR (1993)

Abdel-Mawgood, Ahmed L.

Springer

Biotechnology techniques 7 (1993), S. 703-706

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ISSN: 1573-6784

Source: Springer Online Journal Archives 1860-2000

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: Summary A procedure is presented for using PCR to screen individual colonies, representing clones obtained after controlled unidirectional exonuclease III digestion, for the sizes of the individual inserts. Many clones can be screened at one time since the plasmid templates used for amplification need not be purified but are prepared from individual colonies by a simple procedure that takes less than 30 minutes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00152616

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