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Digitale Medien

Method to identify hexahistidine fusion proteins in crude cellular lysate (1994)

Piesecki, Susan ; Hochuli, Erich

Springer

Biotechnology techniques 8 (1994), S. 39-44

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Details

ISSN: 1573-6784

Quelle: Springer Online Journal Archives 1860-2000

Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Summary The strong interaction of chelated nickel ions with a hexahistidine peptide was utilized to identify hexahistidine fusion proteins in crude cellular lysates. The technique involves a Ni2+-nitrilotriacetic acid derivative labelled with alkaline phosphatase. The new reagent was used to probe for hexahistidine fusion proteins after SDS-PAGE electrophoresis and blotting onto nitrocellulose. The minimal amount of fusion protein which could be detected with this method was 0.1 μg.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00207631

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