ISSN:
1573-5060
Keywords:
Cucumis sativus L.
;
rDNA
;
regeneration systems
;
somaclonal variation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract Somaclonal variation in the Borszczagowski line of Cucumis sativus L. was determined for five regeneration systems: micropropagation (MP), direct leaf callus regeneration (DLR), leaf callus regeneration (LCR), recurrent leaf callus regeneration (RLCR), and direct protoplast regeneration (DPR). The frequency at which new phenotypes appeared in R1 lines and the stability of the rDNA region analysed using of five probes were investigated. MP was not subject to change, while DLR caused only infrequent changes. The highest frequency of change arose through DPR (90% of lines) and RLCR (42.8%), as opposed to 5.9% with LCR. Tetraploids were produced only in the case of LCR (4.7%) and RLCR (28%).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018359726626
