ISSN:
1573-5060
Keywords:
passion fruit
;
Passiflora edulis f. flavicarpa
;
Passiflora amethystina
;
somatic hybridization
;
meiotic behavior
;
pollen viabilty
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract Somatic hybrids (SH) obtained by protoplast fusion were investigated for meiotic behavior in order to determine their possible use in breeding programs. Flower buds from four genotypes of SH between P. edulis f. flavicarpa (E) and the wild species P. amethystina (Am) denoted SH (E + Am) # 12, # 13, # 28 and # 35 were collected and the microsporocytes analysed. Meiotic phases showed the presence of abnormalities such as univalents, bivalents and quadrivalents, laggard chromosomes and anaphase bridges. At least 14 bivalents were observed in most of the cells of the hybrid plants. The percentage of cells with quadrivalents ranged from 73.3 in (E + Am) # 13 to 93.3 in (E + Am) # 28 and # 35. Analysis of pollen viability (V) indicated V = 88.23% for # 12, V = 83.86% for # 13, V = 84.20% for # 28, and V = 72.90% for # 35, and fruit development was observed. The high pollen viability, together with the multivalent pairing observed in the four somatic hybrids indicates that these materials can be used for the genetic improvement of yellow passion fruits aiming at introgressions of genes for resistance to diseases and pests.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1003099709021
