ISSN:
1573-5087
Keywords:
cell culture
;
auxins
;
cytokinins
;
confocal laser scanning microscope
;
protoplasts
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract The model system presented here is based on immobilised single cells, derived directly from tobacco mesophyll protoplasts. It allows the adequate steering of cell populations towards expansion, cell cycling or cell resting. Using this approach cells always have the same predictable response to auxins and cytokinins whatever their actual physiological status. This model system opens new ways to study cellular parameters governing these hormone responses, some of which have been explored so far; a) the cytokinin response can equally well be induced by endogenous as by exogenous cytokinins; b) at least two intracellular components, microtubuli and the ER, adapt their architecture to the hormone-induced status of the cell; c) addition of NAA to the cells does not induce a change in the cytoplasmic pH.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00028500
