ISSN:
1573-5117
Keywords:
Enteromorpha
;
genetic engineering
;
micro spectrophotometry
;
parasexual fusion
;
seaweeds
;
Ulvaria
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Enteromorpha linza and Ulvaria oxysperma in North Carolina reproduce exclusively by asexual zoospores. Calcofluor white staining indicated that newly released zoospores lack significant cellulose cell wall material, making them suitable for treatment as protoplasts in a parasexual fusion process using high pH-Ca2+, PEG and centrifugation. Presumptive fusion products were identified by their larger size, twin chloroplasts and eyespots, and presence of fluorescence labelled and unlabelled portions. Parasexual fusion and karyogamy were confirmed by elevated levels of nuclear DNA in fusion cell germlings. In addition, aceto-orcein staining of fusion cell products revealed a diploid chromosome complement of 2N = 20 in Enteromorpha linza. Fusion cells were isolated by killing the more numerous adjacent unfused zoospores with 2-3 min exposure to blue light (410–490 nm). Unexposed fusion cells could be readily distinguished and recovered by micropipette at the 10-day stage.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00040227
