ISSN:
1573-5117
Keywords:
Algal chlorophyll
;
liquid/liquid partitioning
;
chlorophylla
;
pheophytina
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A simple liquid/liquid partitioning procedure was developed which employed aqueous acetonitrile and hexane, for the isolation of chlorophyll and pheophytin. This procedure separated these pigments from other interfering pigmented compounds. The efficacy of this solvent separation method was evaluated using commercially available chlorophylla, b, pheophytina, b, carotenoids, and algal pigment extracts. The recovery efficiencies of this solvent partitioning process for chlorophyll a and pheophytina have been shown to be 95–98% and 93–96%, respectively, furthermore, the chlorophylla fraction was practically free of any contaminating pigments. It appears that a more accurate assessment of chlorophylla and pheophytina can be accomplished employing liquid/liquid partitioning than with the present standard method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00686136
