ISSN:
1588-2780
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Energy, Environment Protection, Nuclear Power Engineering
Notes:
Abstract This is a comparative study of different chromatographic techniques [gas-liquid (GLC), thin-layer (TLC), liquid (LC), high-pressure liquid (HPLC) chromatography] as applied to the analysis and preparative purification of tritium-labelled eicosanoids with a molar radioactivity of 1.8–8.8 TBq/mmol obtained by selective hydrogenation and by chemical or enzymic methods. We demonstrate the possibility of analyzing reaction mixtures and isolating individual multiply labelled eicosanoids with a chemical and radiochemical purity of 95–98%. Special features of HPLC for high molar radioactivity eicosanoids are considered.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02041436
