ISSN:
1588-2780
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Energy, Environment Protection, Nuclear Power Engineering
Notes:
Abstract Radioimmunoassays (RIA) for the determination of the individual lactate dehydrogenase (LDH) isoenzymes, LDH-1 and LDH-2 have been developed. LDH-1 can be measured in the range of 5–100 ng and LDH-2 in the range of 5–80 ng, if there is no significant cross reactivity. Immunization of several rabbits with LDH-1 and LDH-2 isoenzymes reveals that some animals do not produce antisera to LDH-2 while those injected with LDH-1 generated antiserum in each case. The results of the binding studies suggest that a 50% binding that is recommended for RIA can be achieved with a titer value of 1∶2000 dilution of the antisera. Cross reactivity studies indicate that LDH-1 cross reacts with the antisera to LDH-2 if its concentration is higher than 30 ng/ml of the RIA mixture while LDH-2 cross reacts with the antisera to LDH-1 only if its concentration exceeds 80 ng/ml.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02065396
