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  • 1
    Electronic Resource
    Electronic Resource
    Methodology for cell transformation assays with C3H/10T1/2 mouse embryo fibroblasts (1986)
    Springer
    Methods in cell science 10 (1986), S. 165-172 
    Details
    ISSN: 1573-0603
    Keywords: transformation ; fibroblast ; C3H/10T1/2 ; mouse ; carcinogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Basic cell culture conditions required for execution of cell transformation studies with C3H/10T1/2 mouse embryo fibroblasts are described. These protocols permit assessment of the ability of chemicals to convert nontumorigenic C3H/10T1/2 cells to a tumorigenic phenotype characterized by altered controls upon in vitro cell division. Recent assay modifications that enhance the sensitivity of this focus formation assay and permit the study of multistage transformation processes are noted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01405079
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