ISSN:
1573-0832
Keywords:
Antimycotics
;
Biosynthesis of macromolecules
;
Protoplasts
;
Radioactive precursors
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The effects of nine antimycotics on the biosynthesis of cellular macromolecules were analyzed using the regenerating system of protoplats ofAspergillus niger. The incorporation of several specific radioactive precursors into major cellular components were measured in the presence or in the absence of respective agents. Miconazole, ketoconazole, and tolnaftate inhibited the lipid synthesis. 5-Fluorocytosine strongly inhibited the DNA and protein syntheses. Griseofulvin, however, specifically inhibited the synthesis of cell wall polysaccharides, i.e. chitin and glucan. Other agents showed non-specific inhibition effects. The significance of morphological change of hypha as an indicator of antimycotic action and its feasibility as a screening tool for novel antimycotic compounds are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01103473
