ISSN:
1573-5028
Keywords:
Ti plasmid
;
Hygromycin
;
transformation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We show here that plant cells are sensitive to the antibiotic hygromycin-B4. We also show that a chimaeric gene consisting of the nopaline synthase (nos) gene regulatory elements and the E. coli derived hygromycin phosphotransferase (hpt) gene, when transferred to plants' cells, confers resistance to hygromycin B. The chimaeric nos-hpt gene enables efficient selection of DNA transfer to plant cells when used in conjunction with Ti plasmid-derived binary vectors in cocultivation experiments.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00020627