ISSN:
1573-5044
Keywords:
callus-type
;
plant regeneration
;
Puccinellia distans
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Callus was induced from seeds of Puccinellia distans (L.) Parl. on MS medium supplemented with 2 mgl-1 2,4-dichlorophenoxyacetic acid and 0.5 mgl-1 kinetin. Morphogenesis initiation was achieved during subculture on medium containing 0.1 mgl-1 2,4-D. From the point of morphogenetic capacity, 3 types of callus were selected. High frequency of plant regeneration was obtained by selection of embryogenic type of callus, and culture on N6 medium and N6 medium supplemented with kinetin (5–10 mgl-1), or kinetin (2 mgl-1) and IAA (0.5 mgl-1). A high ratio of albinos among regenerants was observed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00047745
