ISSN:
1573-5044
Keywords:
protoplasts
;
agarose
;
Nicotiana tabacum
;
Nicotiana plumbaginifolia
;
Daucus carota
;
Hyoscyamus muticus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Media solidified with agarose resulted in higher plating efficiency of protoplasts than commonly used agar media. Improved culture efficiency was observed with mesophyll protoplasts of Nicotiana tabacum and N. plumbaginifolia and with protoplasts isolated from cell lines of Daucus carota, Hyoscyamus muticus and two lines of N. tabacum. The improvement with agarose was consistent over a wide cell density range and also for different media. The positive effect was not due to the lower temperature at which the protoplasts could be plated. Culture experiments with mixtures of different agar types generally gave intermediate division frequencies. There was no obvious effect on plating efficiency in experiments where diffusion was permitted between agar and other agar types.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00033560
