ISSN:
1573-5044
Keywords:
enzyme purification
;
plant regeneration
;
protoplast culture
;
Solanum dulcamara L. (bittersweet woody nightshade) cell suspensions
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Large populations of viable protoplasts were released from suspension cultured cells of the woody medicinal plant Solanum dulcamara (bittersweet, woody nightshade) when the cells were harvested 3 to 7 months after culture initiation and 4 to 5 days after transfer to fresh medium. A Bio-Gel p6 purified enzyme mixture enhanced the protoplast plating efficiency 6 fold compared to the unpurified mixture, without affecting protoplast yield. Agarose-solidified medium markedly improved protoplast division and colony formation, and enabled protoplasts to be plated at lower densities than in liquid medium. All protoplast-derived tissues produced shoots on MS based medium with 1.0 mgl-1 zeatin. Shoots rooted readily on medium lacking phytohormones. Cytological examination revealed high chromosome stability of suspension cultured cells, of plants derived from such cells, and of protoplast-derived plants. The implication of these results is discussed in relation to the genetic manipulation of this pharmaceutically important plant.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00043687
