ISSN:
1573-5044
Keywords:
albino
;
aspen
;
chimera
;
callus culture
;
somaclonal variation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Pigment as well as isozyme variations were observed among aspen (Populus tremuloides Michx.) plants regenerated from callus cultures. Out of more than 600 plantlets, two chimeric plants (one with green base and two albino shoots and the other with an albino shoot) were produced. Callus derived from albino shoots produced albino as well as chimeric plants when transferred to shoot inducing medium. Isozyme patterns of 119 plants were examined by starch gel electrophoresis. Thirty plants showed variation in shikimic dehydrogenase isozyme and 41 in isocitric dehydrogenase. Variation was also observed in malate dehydrogenase and phosphoglucose isomerase. No variation was seen in 6-phosphogluconate dehydrogenase. Pigment variation was not associated with any isozyme changes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00116087
