ISSN:
1573-5044
Keywords:
Brassica napus
;
protoplasts
;
purification
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The effects of three different general purification protocols have been assessed quantitatively using mesophyll protoplasts of Brassica napus. Within the initial sample two distinct sub-populations were determined. The methods used influenced the ratio of the vacuolated to chloroplastic type protoplast sub-populations. Overall recovery rates of the initial sample varied according to the method used from 38% to 27%, but the relative recovery of the sub-populations varied considerably with a purified ratio of between 1.0:0.78 to 1.0:7.0. Size distribution profiles of the initial and purified populations are also presented.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00044264
