ISSN:
1573-5044
Keywords:
alfalfa
;
somatic embryogenesis
;
germplasm
;
genotypic variation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Seventy-six cultivars of alfalfa (Medicago sativa L., M. falcata L. and M. varia Martyn) were tested in vitro for their capacity to produce callus and somatic embryos. A three-step media protocol was used to survey the response of the cotyledons and hypocotyl of each genotype while the epicotyl region was conserved in order to recover highly responding genotypes. The best regeneration response was observed in creepingrooted cultivars which contained a strong genetic contribution of two landrace germplasm sources, defined as M. falcata and Ladak, in their ancestry. The callus and embryogenesis responses showed a high degree of variation both between cultivars and among the plants of many of the 76 cultivars tested. A higher number of plants produced somatic embryos in the high regenerating cultivars compared to the low regenerating cultivars regardless of the media protocol or explant.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00042269
