ISSN:
1573-5044
Keywords:
Hordeum vulgare
;
plasmid uptake
;
polyethylene glycol (PEG)
;
protoplasts
;
transient gene expression
;
stable transformation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cat gene expression has been investigated following PEG-mediated plasmid uptake into barley protoplasts. The uptake conditions optimised for transient expression were employed for stable transformation. Transformed protoplast-derived calli of the cvs. Dissa and Igri, were selected on medium containing G418 at 40 μg ml−1 or kanamycin sulphate at 250 μg ml−1. Absolute transformation frequencies of 28.9×10−5 and 21.3×10−5 were recorded for Dissa with kanamycin sulphate and G418 selection, respectively. The frequency for Igri was 11.5×10−5 with G418 selection. Antibiotic resistant protoplast-derived colonies expressed NPTII activity; Southern hybridisation confirmed integration of the nptII gene into barley genomic DNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00051581
