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Rapid plant regeneration of pea using thidiazuron (1996)

Sanago, Massimo H. M. ; Shattuck, Vern I. ; Strommer, Judith

Springer

Plant cell, tissue and organ culture 45 (1996), S. 165-168

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ISSN: 1573-5044

Keywords: organogenesis ; Pisum sativum ; regeneration ; tissue culture

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A simple and rapid pea regeneration procedure was developed. An average of up to 20 shoots formed from hypocotyl explants of cvs. Sugar Ann and Patriot cultured on Murashige and Skoog basal medium supplemented with 0.5 or 1.0 μM thidiazuron (N-phenyl-N′-1,2,3-thiadiazol-5′-ylurea). Hypocotyls of Puget and Sugar Daddy did not respond. Regenerated shoots rooted rapidly when cultured on Murashige and Skoog basal medium containing either 2.0 μM α-naphthaleneacetic acid or 1.0–2.0 μM indole-3-butyric acid. Seeds were harvested from regenerated plants after only 9–11 weeks.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00048761

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