Electronic Resource
Springer
Veterinary research communications
5 (1981), S. 183-185
ISSN:
1573-7446
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract A simple and reproducible method of establishing cell lines from the blood of sheep and cattle is described. Buffy coat cells were allowed to adhere to plastic culture flasks in media containing 20 per cent autologous plasma overnight. The fluids were then replaced with growth medium supplemented with non-inactivated foetal calf serum, lamb serum or autologous serum. Ovine cell lines were established with any of the serum supplements but bovine cell lines were established more readily if unheated autologous serum was used.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02214984
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