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Hydroxylation in isolated liver cells (1975)

Kanaeva, I. P. ; Archakov, A. I.

Springer

Bulletin of experimental biology and medicine 80 (1975), S. 1193-1194

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ISSN: 1573-8221

Keywords: isolated cells ; hydroxylation ; oxidative phosphorylation ; oxidation

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract Isolated cells were obtained by using EDTA and mechanical action to dissociate rat liver tissue. The presence of oxidative phosphorylation reactions in the hepatocytes was taken as an indication of their integrity. The hepatocytes were able to hydroxylate dimethylaniline, ethylmorphine, and aminopyrine. The maximal velocity of hydroxylation was higher in the cells than in microsomes, when calculated per nmole cytochrome P-450. The NAD·H2 formed by oxidation of glutamate and malate in isolated cells can be used for the hydroxylation reactions.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00833157

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