ISSN:
1573-4935
Keywords:
bacteriorhodopsin
;
retinal
;
cation binding sites
;
proteolysis
;
bleached membrane
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Bleaching of the purple membrane strongly reduces the number of divalent cation binding sites as well as their affinities. Conversely, deionization of the bleached membrane drastically inhibits the chromophore regeneration. Proteolysis experiments using bromelain show that the bleached membrane has an additional cleavage site probably located at the fifth loop, whereas in the blue membrane, the C-terminal tail is no longer susceptible to proteolysis. It is suggested that there exists a close relationship between the retinal environment and one or more of the cation binding sites.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01114972
