ISSN:
1573-7217
Keywords:
aneuploidy
;
breast cancer
;
flow cytometry
;
proliferative rate
;
quality control
;
S-phase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Consecutive sections from 33 paraffin-embedded human breast carcinomas without intratumor heterogeneity were sent for flow cytometric (FCM) DNA analysis in two experienced laboratories. FCM instruments, run conditions, and tumor disaggregation procedures were different in the two laboratories. In four cases (12%) the laboratories reported a different DNA ploidy and DNA index (DI). These variations were due to analytical reasons, differences in the detection rates of near-diploid and tetraploid DIs, not due to interpretation of data or the criteria used for aneuploidy. There was a significant correlation between S-phase fractions (SPF) obtained in the two laboratories (r = 0.90, p〈0.0001) if only cases with concordant DI were included. Discordant DI usually led to very different SPF values.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01812686
